DM 45 Publications:

Key Publications

  • “Cytochrome b5 Binds Tightly to Several Human Cytochrome P450…”
    Uses fluorescence titrations with b₅ conjugated probe carried out in an OLIS DM45 spectrofluorometer (“A solution of 50 nM Alexa 488‑T70C‑b₅ … placed … in an OLIS DM45 spectrofluorometer”) scholarworks.utep.edu+15PMC+15ScienceDirect+15.

  • “Blue light induces major changes in the gene expression profile of …”
    Reports: “Fluorescence at 77 K was measured … with an OLIS DM45 spectrofluorometer …” PMC+1SpringerLink+1.

  • Kinetic analysis of oxidation of coumarins by human cytochrome
    Specifies use of an OLIS DM45 spectrofluorimeter for kinetic fluorescence traces ScienceDirect+1JBC+1.

  • Proteomics, modeling, and fluorescence assays delineate …
    Fluorescence spectra (493 nm excitation, 500–600 nm emission) were obtained with an OLIS DM‑45 spectrofluorimeter, slits 1.24 mm ScienceDirect+15PMC+15PMC+15.

  • Kinetics and Thermodynamics of Ligand Binding by Cytochrome …
    Fluorescence measurements performed using a DM45/OLIS spectrofluorimeter ScienceDirect+3JBC+3JBC+3.

  • Carbohydrate based biomarkers enable hybrid near infrared …
    Emission measured by “OLIS SLM8000 or OLIS DM 45” spectrometer OLIS CLARiTY+3PMC+3OLIS CLARiTY+3.

  • Steady-state fluorescence experiments in DNA repair studies
    Cites: “Fluorescence assays were done using an OLIS DM45 spectrofluorometer (On‑Line Instrument Systems, Bogart, GA)” ScienceDirect+15ScienceDirect+15PMC+15.

  • Binding of cytochrome P450 27C1 to its substrate
    Reports use of an OLIS DM‑45 spectrofluorometer, 1.24 mm slits, 493 nm excitation PMC+3ScienceDirect+3PMC+3.

  • Antioxidant intervention in radical‑induced protein damage (thesis)
    Equipment: “DM45 Olis fluorescence spectrometer, equipped with a 75 W Xenon arc lamp” Canisius University+2scholarworks.utep.edu+2Labcompare+2.

  • Biochemistry teaching lab project-based paper
    Notes: “An OLIS DM 45 spectrofluorometer with scanning excitation/emission monochromators (5 nm bandpass slits)” IUBMB Life+1Canisius University+1.

  • Effects of oxidation on protein–nanoparticle interactions
    Used “an OLIS DM45 spectrofluorimeter and a 10 mm cuvette” for fluorescence quenching digitalcommons.csbsju.edu.

  • Liver fatty acid binding protein FABP1 study (2025)
    Buffer baseline recorded using an OLIS DM‑45 spectrofluorometer (1.24 mm slit, 470 nm excitation) ScienceDirect.

🧩 Summary

The OLIS DM‑45 is widely used in:

  • Fluorescence titration and kinetics for enzyme–ligand interactions (cytochromes, coumarins)

  • Low-temperature (77 K) fluorescence in photosynthetic organism studies

  • Fluorescence spectroscopy in DNA repair, nanoparticle–protein, and biomarker research

  • Educational labs for teaching fluorescence principles

Consistently referenced across biochemical, biophysical, and environmental studies, it remains a reliable and trusted instrument in academic settings.