For peer-reviewed publications, click here.
For peer-reviewed publications, click here.
Example Applications for a CLARiTY UV/Vis
What would yours be?
Broadband growth / concentration monitoring of intact cells for accurate biomass quantitation even with scattering; seeing how the pigment profile changes during growth to track physiological state, not just concentration; real-time, non-destructive monitoring from fermenters, photobioreactors, cell factories; and much more!
Pigment quantitation in intact cells (chlorophyll, carotenoids, heme) to capture specific absorbance peaks at precise wavelengths (e.g., his algal culture has 12 µg/mL chlorophyll a and 3 µg/mL carotenoids; the heme content of this bacterial suspension doubles during stationary phase; etc.
Aggregation kinetics of nanoparticles or formulations (is the drug nanoparticle formulation stable or prone to clumping), useful while adjusting stabilizers or surfactants to reduce aggregation; provides a quantitative measure of shelf-life or batch consistency.
Drug loading & release in nanoparticle formulations (in situ), i.e., how quickly the drug is taken up by the nanoparticles during loading, or how fast it is released into the surrounding medium; the loading efficiency (% of drug actually loaded into nanoparticles); measure in the natural state of the formulation, avoiding artifacts caused by sample manipulation; and much more!
Kinetics of cell lysis or membrane permeabilization, i.e., monitor cell viability during protein production in bioreactors to maximize yields; evaluate how nanoparticles, peptides, or drugs affect cell membranes; screen compounds for cytotoxicity quickly and quantitatively.
Protein corona formation and binding on nanoparticles (spectral shift & scattering changes), i.e., rate of corona formation, saturation point (maximum protein coverage), stability of the corona over time, etc.
Enzyme assays with particulate substrates, i.e., design better enzymes, formulations, and reaction conditions; multiple enzyme-substrate combinations can be monitored without tedious sample prep; real-time monitoring of biocatalytic processes (biofuels, food processing, detergents) improves efficiency and quality control.